Journal: Neuron
Article Title: Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients
doi: 10.1016/j.neuron.2022.11.007
Figure Lengend Snippet: Infection of the respiratory mucosa with Delta, Omicron BA.1, or Omicron BA.2 Confocal images of sections through the respiratory mucosa of COVID #87 (Delta), #95 (Omicron BA.1), #104 (Omicron BA.1), #101 (Omicron BA.2), and #107 (Omicron BA.2) stained with RNAscope and IHC. Names of genes (italics) and proteins (roman) are at the bottom left, scale bars at the bottom right. DAPI served as nuclear stain. (A) N puncta are densely packed in cells harboring FOXJ1 puncta or containing KRT8-IR signal. (B) Nucleocapsid-IR signal is widespread. N-sense puncta and orf1ab-sense puncta reflect ongoing viral replication. (C) MUC5AC-IR mucus covers a stretch of respiratory epithelium with N-sense puncta in cells harboring FOXJ1 puncta. (D) S-sense puncta occur at a characteristic perinuclear position in nucleocapsid-IR cells. (E) Nucleocapsid-IR signal outlines the contours of ciliated cells, which harbor densely packed S puncta. (F) At the apical edge of a crypt or gland duct, KRT8-IR cells harbor N puncta. Deeper, clusters of extracellular N puncta reflect virions released from cells. (G) The dashed square in (F) imaged at higher magnification. (H) In a section near (G), extracellular S puncta colocalize with nucleocapsid-IR signal. (I and J) Omicron BA.2 (I) or Omicron BA.1 (J) swept through a broad swath of the respiratory epithelium, with nearly every nucleocapsid-IR cell harboring N-sense puncta and orf1ab-sense puncta.
Article Snippet: RNAscope Hydrogen Peroxide and Protease Plus , Advanced Cell Diagnostics , Cat# 322330.
Techniques: Infection, Staining, RNAscope, Data-independent acquisition